Animal Products

Catalytic Mechanisms of Metmyoglobin on the Oxidation of Lipids in Liposome Model System

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Abstract

The catalytic mechanism of metmyoglobin (metMb) on the development of lipid oxidation in a phospholipid liposome model system was studied. Liposome model system was prepared with metMb solutions (2.0, 1.0, 0.5, and 0.25 mg metMb / mL) containing none, diethylenetriamine pentaacetic acid (DTPA), desferrioxamine (DFO), or ferric chloride and lipid oxidation was determined at 0, 15, 30, 60, and 90 min of incubation at 37 °C. Metmyoglobin catalyzed lipid oxidation in the liposome system, but the rate of lipid oxidation decreased as the concentration of metMb increased. The amount of free ionic iron in the liposome solution increased as the concentration of metMb increased, but the rate of metMb degradation was increased as the concentration of metMb decreased. The released free ionic iron was not involved in the lipid oxidation of model system because ferric iron has no catalytic effect without reducing agents. Both DFO and DTPA showed antioxidant effects, but DFO was more efficient than DTPA because of its multifunctional antioxidant ability as an iron and hematin chelator and an electron donor. The antioxidant activity of DTPA in liposome solution containing 0.25 mg metMb/mL was two times greater than that with 2 mg metMb/mL due to the increased prooxidant activity of DTPA-chelatable compounds. It was concluded that ferrylmyoglobin and DTPA-chelatable hematin generated from the interaction of metMb and LOOH, rather than free ionic iron, were the major catalysts in metMb-induced lipid oxidation in phospholipid liposome model system.

Keywords: ASL R2680

How to Cite:

Min, B., Nam, K. & Ahn, D. U., (2012) “Catalytic Mechanisms of Metmyoglobin on the Oxidation of Lipids in Liposome Model System”, Iowa State University Animal Industry Report 9(1). doi: https://doi.org/10.31274/ans_air-180814-1045