Poultry

Host-Pathogen Protein-Protein Interaction Prediction Using an in silico Model

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Abstract

Newcastle Disease (ND) is caused by Newcastle Disease Virus (NDV) and is a major problem in developing countries where vaccination against NDV is not easily achievable. A step required for NDV infection is the cleavage of the NDV fusion (F) protein. Using structural information of the NDV F protein and the only known host protein binding partner, protein disulfide isomerase A3 (PDIA3), we computer modeled the interaction between the two proteins by looking at a docked structure of these two proteins. With our docked structure, we visualized one of the catalytic domains of PDIA3 being near the cleavage site of the NDV F protein. We also discovered a novel binding pocket on the NDV F protein that interacts with the second catalytic domain of PDIA3. This new insight may provide additional molecular targets for NDV vaccine development.

Keywords: Biochemistry Biophysics and Molecular Biology

How to Cite:

Hsieh, J. C., Jernigan, R. L. & Lamont, S. J., (2016) “Host-Pathogen Protein-Protein Interaction Prediction Using an in silico Model”, Iowa State University Animal Industry Report 13(1). doi: https://doi.org/10.31274/ans_air-180814-224