Animal Health

Immunization for Influenza A Virus by Intranasal Administration of Alphavirus Replicon Particles

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Abstract

Improved vaccines are necessary to prevent swine influenza, especially in young growing pigs. The objective of this study is to determine whether intranasal vaccination with Alphavirus replicon particle (RP) vector vaccine prevents influenza A virus (IAV) in pigs. RP vaccine was prepared with the hemagglutinin (HA) gene of pandemic H1N1 influenza virus (A/California/04/2009, pH1N1). The efficacy of intranasal (IN) administration with pH1N1 HA RP was evaluated in two pig experiments. In the first experiment, prime/boost RP vaccination was administered IN/IN to pigs. In the second experiment, pigs were administered a one dose intramuscular (IM) or IN HA RP vaccine, or with a combination of IN/IM routes with an interval of three weeks. Results showed that two doses IN administration of HA RP did not protect pigs against IAV; one dose IM and combination IN/IM routes vaccination with HA RP reduced pneumonia significantly and partially inhibited virus shedding following homologous challenge.

Keywords: ASL R2750

How to Cite:

Chen, Q., Vander Veen, R., Madson, D. M. & Harris, D. H., (2013) “Immunization for Influenza A Virus by Intranasal Administration of Alphavirus Replicon Particles”, Iowa State University Animal Industry Report 10(1). doi: https://doi.org/10.31274/ans_air-180814-29