Reciprocal Meat Conference Abstracts

Impact of Fat Content of Beef on Heat Resistance of Non-O157:H7 Shiga Toxin Producing Escherichia Coli

---

Abstract

ObjectivesTo quantify the effect of fat content on heat resistance of non-O157:H7 Shiga toxin producing Escherichia coli (STEC) in ground beefMaterials and MethodsThe thermal inactivation of six strains of non-O157 group, Escherichia coli: O26:H1, O45:H2, O103:H2, O111:H8, O121:H9, and O145: non-motile was studied. Previously, we studied the decimal reduction values (D-values) of non O157:H7 STEC strains individually in Tryptic soy broth (TSB). Data indicated no significant differences (p > 0.05) in the D-values among the strains at 55, 60, and 65°C. Hence, we used a cocktail mixture of these strains to calculate D-values in ground beef. For this study, six fat levels 5, 10, 15, 20, 25, and 30% of ground beef were obtained and stored at -20°C,; thawed at 4°C for 24 h before use. Nalidixic acid (NAL) resistant strains were used in the study to differentiate background flora of meat. Bags containing 5 g of inoculated ground beef were submerged in a circulating water bath set at 3 temperatures- 55, 60, and 65°C. Samples were taken out from the water bath at fixed time interval (7.5 min at 55°C, 30 s at 60°C and 5 s at 65°C) and surviving populations was enumerated by plating onto plate count agar (PCA) supplemented with 50-ppm of NAL. Survival curve was plotted between the log survival population and exposure time with a correlation coefficient (r2) > 0.9. D-value was calculated by taking the negative inverse of the slope of the survival curve. All experiments were performed in triplicate.ResultsA log-linear decline was observed in the population of STECs by heating of ground beef. The D55°C in 5% fat was 15.93 min. and a significant decrease (p < 0.05) to 12.66 min. at 25% was observed, which further decreased significantly (p < 0.05) to 11.69 min. at 30% fat content. There were no significant differences (p > 0.05) in other fat levels at 55°C. However, increase in fat content did not have a significant impact (p > 0.05) on D60°C and D65°C. The D60°C were 1.15, 1.16, 1.10, 1.10, 0.91, 1.12 min and D65°C were 0.16, 0.14, 0.12, 0.12, 0.12, 0.08 min at 5, 10, 15, 20, 25, and 30% fat content, respectively. The z-values were 5.07, 5.16, 4.94, 4.96, 4.94, and 4.62°C for 5, 10, 15, 20, 25, and 30% fat contents, respectively.ConclusionA negative correlation was observed between the D-values of non-O157 STECs and fat content, indicating more kill rate with high fat content of ground beef at 55°C. The impact of fat level on D-value of non-O157 STECs is not available in the literature. The results provided are new finding and will help to successfully eliminate non-O157 STECs from cooked and ready-to-eat meat products

Keywords: Big Six E. coli, STEC, meat safety, D-value, ground beef

How to Cite:

Brar, J. S., Bailey, M., Corkran, S., Velasquez, C. & Singh, M., (2018) “Impact of Fat Content of Beef on Heat Resistance of Non-O157:H7 Shiga Toxin Producing Escherichia Coli”, Meat and Muscle Biology 1(2). doi: https://doi.org/10.221751/rmc2016.118